Metabolic responses of clams, ruditapes decussatus and. Oxidative stress and damage biomarkers in clam ruditapes. Isolated from the clams ruditapes philippinarum and ruditapes decussatus. Illumina reads from all 12 samples were pooled and compared to a set of 20 bivalvia mitochondrial genomes to identify reads with mitochondrial origin. Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam ruditapes decussatus larvae aquaculture international, aug 2016 amel medhioub, badreddine mechri, sondes bchir, younes limeyem, wissem slimani, mahjoub aouni, mohamed nejib medhioub. In the aquatic environment, they are subjected to the presence of different contaminants, such as mercury hg and its methylated form. May 12, 2011 the manila clam, ruditapes philippinarum, is one of the major aquaculture species in the world and a potential sentinel organism for monitoring the status of marine ecosystems.
Phylogenetic analysis based on 16s rrna gene sequences showed that these marine bacteria were closely related to the recently described species vibrio comitans, vibrio rarus and vibrio inusitatus 99. Download fulltext pdf microbiological monitoring of ruditapes decussatus from ria formosa lagoon south of portugal article pdf available in journal of coastal conservation 173. Pdf metallothioneins in the clam ruditapes decussatus. Analysis of the 16s rrna gene allowed the identification of the strains as members of the genus marinomonas, sharing the highest similarity with marinomonas. A study of gonadal development in ruditapes decussatus l. Icpoes analysis was further performed on the clams. Mussels from a control site were transplanted during 3, 15 and 30 days into.
A total of 146 immunerelated genes were identified in the transcriptome of the mantle of r. This study investigated the effects of 48h heavy metal exposure upon the metabolic profiles of ruditapes decussatus and ruditapes philippinarum using 1h nmr metabolomics. Download fulltext pdf download fulltext pdf behaviour and biochemical responses of the marine clam ruditapes decussatus exposed to phosphogypsum article pdf available in environmental. In the present study, a multibiomarker approach was used to assess the biological effects of metal pollution in the southern lagoon of tunis, on clam ruditapes decussatus both in hot in summer and cold in winter seasons. Topics covered include microbiology, botany, zoology, ecosystem research, biological oceanography, ecological aspects of fisheries and aquaculture, pollution, environmental protection, conservation, and resource management. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer, from where it. All structured data from the file and property namespaces is available under the creative commons cc0 license. A previous analysis on the cox1 gene of ruditapes decussatus reported a nucleotide diversity. The grooved carpet shell clam ruditapes decussatus is the autochthonous european clam and the most appreciated from a gastronomic and economic point of view.
Download this pdf file the pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader. Uncovering the immunological repertoire of the carpet shell. Pdf microbiological monitoring of ruditapes decussatus. This means that randomly sampled individuals of ruditapes decussatus used in this work showed a much lower mtdna variability than ruditapes philippinarum siblings. The editorial office will send you the word version of the file and i append a pdf version of it here, for the record. Review history for the complete mitochondrial genome of the. Transcriptome sequencing and microarray development for the. Both species were exposed to increasing concentrations of lead nitrate 10, 40, 60 and 100. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader if you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs alternatively, you can download the pdf file directly to your computer. Preliminary study of the clam ruditapes decussatus exposed in. Pdf microbiological monitoring of ruditapes decussatus from.
The harvesting of ruditapes decussatus mainly occurs in spain and france. We studied the expression of pigst, segpx, and mt transcripts in the digestive gland of dreissena polymorpha exposed to organic and metallic pollutants. Virus present in the connective tissues sometimes larvae and juvenile mortalities. If you would like more information about how to print, save, and work with pdfs, highwire press provides a helpful frequently asked questions about pdfs. The complete mitochondrial genome of the grooved carpet. This study focused on industrial effluent characterization and determination of its effects on the clam ruditapes decussatus. The production is in decline due to several factors such as perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam ruditapes. Bearded horse mussel modiolus barbatus and noahs ark shell arca noae are a species of interest for the diversifying shellfish aquaculture on the southeastern coast of the adriatic.
Change in fatty acid composition and evaluation of lipids. Facultative anaerobic marine bacteria isolated from cultured clams ruditapes decussatus and ruditapes philippinarum were previously investigated using aflp and 16s rrna gene sequences analyses. Ruditapes philippinarum and ruditapes decussatus under hg. Apr 14, 2015 the native species ruditapes decussatus and the invasive species ruditapes philippinarum have an important ecological role and socioeconomic value, from the atlantic and mediterranean to the indopacific region. Download fulltext pdf download fulltext pdf morphometric analysis of european clam ruditapes decussatus in morocco article pdf available in aacl bioflux 125. Parasite present in connective tissue and epithelial cells. In the aquatic environment, they are subjected to the presence of different contaminants, such as mercury hg and its methylated form, methylmercury mehg. Introduced ruditapes philippinarum have become naturalized in many areas of europe, competing with native r. For this reason, interest in the immune genes in this species has recently increased.
Impacts of rearing techniques on growth, survival and. Pdf morphometric analysis of european clam ruditapes. Ruditapes decussatus is morphologically and ecologically similar. It is distributed worldwide and due to its ecological and economic interest has been proposed as a bioindicator this species is one of the most popular and profitable mollusc of lagoonal and coastal sites in the mediterranean, where. Comparative study of gonadal development of ruditapes. The eastern oyster crassostrea virginica, the carpet shell clam ruditapes decussatus, the manila clam ruditapes philippinarum, the pacific oyster crassostrea gigas and several species. The isolates formed two aflp clusters and belonged to the genus vibrio, more precisely to the splendidus clade. Metabolic responses of clams, ruditapes decussatus. Files are available under licenses specified on their description page. Seven nonmotile, facultatively anaerobic, alginolytic marine bacteria were isolated from the cultured clams ruditapes philippinarum and ruditapes decussatus. The regional government of galicia spain has banned the use of manila clam seed for semiextensive use on beaches, and several authorities are.
We have studied differential gene expression related to differences in growth rate in adult individuals of the commercial marine clam ruditapes decussatus. This page was last edited on 26 december 20, at 12. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various environmental stressors on. Nov 01, 2003 read preliminary study of the clam ruditapes decussatus exposed in situ to metal contamination and originating from the gulf of gabes, tunisia, bulletin of environmental contamination and toxicology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Genoyster 20152018 validation of dnaparentage assignment panel for pacific oyster c. Pdf behaviour and biochemical responses of the marine. A biomonitor of trace elements pollution in sardinian coastal lagoons italy.
Glutathione stransferases gst, glutathione peroxidases gpx and metallothioneins mt are essential components of cellular detoxication systems. In this study, oxygen consumption oc, total ammonia excretion tam and clearance rate cr responses to the changes in seawater salinity 37, 30, 25 and 20 were. Ruditapes decussatus, the manila clam ruditapes philippinarum, the pacific oyster crassostrea gigas and several species other clams, abalone, scallops can be tested the same way. Thirty eight percent of the blast hits were obtained with members of the family veneridae followed by 27% with members of the family ostreidae see fig. Comparative transcriptomics in two bivalve species offers.
Introduced into poole harbour for aquaculture in 1988. Naturalization of the manila clam tapes philippinarum. In genes with pdistance 40%, the relationship is better explained by an exponential function red. Phylogenetic analysis based on 16s rrna gene sequences showed that these marine bacteria were closely related to the recently described species vibrio. It is distributed worldwide and due to its ecological and economic interest has been proposed as a bioindicator. Clams were collected in august 2015 and february 2016 from three sites of the lagoon and from louza considered a reference site. Abalone mortalities in australia and clams mortalities in portugal, korea, china, japan. Burrowed in intertidal to sublittoral soft sediments, chiefly in sheltered areas. Gene expression profile analysis of manila clam ruditapes. Research article open access mrnaseq and microarray.
Survivin immunoreactivity in the gastric mucosa of rats feedind with carpet shell clam ruditapes decussatus topics dardanelles, immunohistochemistry, stomach, carpet shell clam, survivin. The mitochondrial genome of ruditapes decussatus was not available in the databases, so we used the transcriptome data to generate a draft to be used as a guide for sanger sequencing. Intensive fishing for clams began in 1926 and 1927. Jun 15, 2016 this study investigated the effects of 48h heavy metal exposure upon the metabolic profiles of ruditapes decussatus and ruditapes philippinarum using 1h nmr metabolomics. In this study, phylogenetic analyses based on sequences of. The pdf file you selected should load here if your web browser has a pdf reader plugin installed for example, a recent version of adobe acrobat reader. In spain, early records of mollusc fishing and consumption from the 16 th century are mainly about the flat oyster, and only rarely about clams, but they do mention the marketing of clams in portugal and other places. Influence of salinity on physiological response of the. The present work describes the construction of the first r. In this study, oxygen consumption oc, total ammonia excretion tam and clearance rate cr responses to the changes in seawater salinity 37, 30, 25 and 20. Download fulltext pdf microbiological monitoring of ruditapes decussatus from ria formosa lagoon south of portugal article pdf available in. The leading journal in its field, meps covers all aspects of marine ecology, fundamental and applied.
The complete mitochondrial genome of the grooved carpet shell. Despite the large number of animal complete mitochondrial genomes currently available in public databases, knowledge about mitochondrial genomics in invertebrates is uneven. In vitro culture of perkinsus atlanticus, a parasite of the carpet shell clam ruditapes decussatus by m. Naturalization of the manila clam tapes philippinarum, an. The main objective of the present study was to gain further insights into the immunological repertoire of r. Transcriptome sequencing and microarray development for. Impacts of rearing techniques on growth, survival and bacterial microbiota of carpet shell clam ruditapes decussatus larvae aquaculture international, aug 2016 amel medhioub, badreddine mechri, sondes bchir, younes limeyem, wissem slimani, mahjoub aouni.
Survivin immunoreactivity in the gastric mucosa of rats feedind with carpet shell clam ruditapes decussatus topics dardanelles, immunohistochemistry, stomach, carpet shell clam. Review history for the complete mitochondrial genome of. Growth rate is one of the most important traits from the point of view of individual fitness and commercial production in mollusks, but its molecular and physiological basis is poorly known. The production is in decline due to several factors such as perkinsiosis and habitat invasion and competition by the introduced exotic species, the manila clam ruditapes philippinarum. A linear function black dashed line describes the relationship between dnds and pdistance in genes with lower pdistance. Despite the large clam harvests, their repopulation is rapid. The first known occurrence of a naturalized population of manila clam tapes philippinarum in uk waters is reported. International journal of systematic and evolutionary microbiology 61 10. Journal of the marine biological association of the united kingdom. Pescados mariscos moluscos pdf 579 1 linkedin slideshare. The grooved carpetshell clam ruditapes decussatus is native to the northern atlantic and mediterranean sea and has a high commercial value. Camino ordas and antonio figueras huerta download pdf 2 mb. In 1948 it was estimated that populations of ruditapes decussatus and venerupis pullastra in the ria del burgo spain recovered in less than a year from a density of 15 to 3050 clamsm 2. Preliminary study of the clam ruditapes decussatus exposed.
Uncovering the immunological repertoire of the carpet. In this study, we characterized the transcriptome of male and female mature gonads in the european clam ruditapes decussatus, compared with that in the manila clam ruditapes philippinarum providing, for the first time in bivalves, information about transcription dynamics and sequence evolution of sexbiased genes. Reproductive pattern of the clams, ruditapes decussatus and r. The grooved carpet shell, or palourde clam, ruditapes decussatus, or venerupis decussatus, is a clam or bivalve mollusc in the family veneridae. Global analysis of gene expression profiles is increasingly used to evaluate the biological effects of various. A new threephase culture method for manila clam, ruditapes philippinarum, farming in northern china. The manila clam ruditapes philippinarum is a cultured bivalve with worldwide commercial importance, and diseases cause high economic losses. Clam stock on the western coast of cotentin english channel the manila clam ruditapes philippinarum adam and reeve 1850 was introduced into france in the 1970s and now dominates the european clam ruditapes decusattus linnaeus 1758 in the intertidal zone of the western cotentin english channel. The native species ruditapes decussatus and the invasive species ruditapes philippinarum have an important ecological role and socioeconomic value, from the atlantic and mediterranean to the indopacific region. Zipcodezoo this veneridaerelated article is a stub. This paper reports, for the first time, the complete mitochondrial genome of the grooved carpet shell, ruditapes decussatus, also known as the european clam.
Scope this procedure explains the diagnostic test used for the protistan perkinsus sp. Change in fatty acid composition and evaluation of lipids and. Pdf the clam ruditapes decussatus is a suspensionfeeding bivalve mollusc widely distributed in european waters and in the mediterranean. It is one of the main native bivalve species cultured in europe.
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